KMID : 0988920200180010115
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Intestinal Research 2020 Volume.18 No. 1 p.115 ~ p.120
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Proinflammatory cytokines in irritable bowel syndrome: a comparison with inflammatory bowel disease
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Mitselou Antigony
Grammeniatis Vasileios Varouktsi Anna Papadatos Stamatis S. Katsanos Konstantinos H. Galani Vasiliki
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Abstract
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Background/Aims: Irritable bowel syndrome (IBS) is a common disease often considered as a functional intestinal disorder. Inflammation in IBS is a quite intriguing theory. The aim of this study was to investigate tumor necrosis factor (TNF)-¥á, interleukin (IL)-1¥â and IL-6 expression in inflammatory bowel disease (IBD) patients, IBS patients and normal controls.
Methods: IBS and IBD patients along with normal controls were recruited in the study. In all groups, 2 pinch biopsies were taken at each of 3 anatomical sites (terminal ileum, cecum, and rectum). IBS patients were also subcategorized according to the syndrome clinical manifestations. Two monoclonal antibodies (mAb), TNF-¥á mAb and IL-6 mAb, and one polyclonal antibody IL-1¥â mAb were applied for immunohistochemical analysis.
Results: In IBD patients intensity of TNF-¥á and IL-1¥â were lower than in IBS patients or controls, while IL-6 was significantly increased comparing to the aforementioned groups. In IBS patients TNF-¥á was increased comparing to IBD patients or controls, while IL-6 and IL-1¥â were similar to controls. In IBS subgroups, TNF-¥á was lower in diarrhea predominant IBS patients and higher constipation predominant IBS patients. Differences among IBS subgroups regarding IL-6 and IL-1¥â were nonsignificant.
Conclusions: IL-6 seems to be the most important proinflammatory cytokine in IBD patients, while TNF-¥á could play a more significant role in IBS pathogenesis.
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KEYWORD
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Proinflammatory cytokines, Irritable bowel syndrome, Inflammatory bowel disease
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